علمی- ترویجی (حرفه‌ای) دامِستیک

علمی- ترویجی (حرفه‌ای) دامِستیک

نقش بربرین و نانو‌بربرین به ‌عنوان آنتی‌اکسیدان در حفظ کیفیت و انجمادپذیری اسپرم دامی: یک مطالعة مروری

نوع مقاله : مقاله علمی- ترویجی

نویسندگان
1 پژوهشگر پسادکتری فیزیولوژی دام، گروه مهندسی علوم دامی، دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران، کرج، البرز، ایران
2 استاد فیزیولوژی دام، گروه مهندسی علوم دامی، دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران، کرج، البرز، ایران
چکیده
در فرآیند انجماد اسپرم، تنش اکسیداتیو یکی از عوامل اصلی کاهش تحرک، زنده‌‌مانی، یکپارچگی غشایی و در نهایت توان باروری اسپرم محسوب می‌‌شود. سلول‌های اسپرم به دلیل حجم اندک سیتوپلاسم، سطح پایین آنتی‌اکسیدان‌‌های درون ‌سلولی، غشای غنی از اسیدهای چرب غیراشباع چند پیوندی و حساسیت بالا به پراکسیداسیون لیپیدی، نسبت به سایر سلول‌‌ها آسیب‌پذیری بیشتری در برابر استرس اکسیداتیو دارند. افزودن آنتی‌اکسیدان‌‌ها به رقیق‌کننده‌‌های انجماد به ‌عنوان یک راهکار مؤثر جهت کاهش آسیب‌های ناشی از شوک سرمایی و فرآیند انجماد–ذوب مورد توجه قرار گرفته است، هر چند پایداری و نیمه‌ عمر این ترکیبات ممکن است در طی فرآیند انجماد کاهش یابد. بربرین، یک آلکالوئید گیاهی با خواص آنتی‌اکسیدانی قوی، به دلیل جذب زیستی پایین با محدودیت کاربرد مواجه است. در این راستا، استفاده از نانو‌بربرین می‌‌تواند از طریق افزایش پایداری، بهبود جذب و افزایش تماس با سلول‌‌های هدف، اثرات حفاظتی مؤثرتری در برابر تنش اکسیداتیو ایجاد کند. بر این اساس، مقاله علمی- ترویجی حاضر به‌ صورت مروری به بررسی نقش آنتی‌اکسیدانی بربرین و نانو‌بربرین در رقیق‌کننده‌‌های انجماد اسپرم و تأثیر آن‌‌ها بر بهبود کیفیت اسپرم منجمد–ذوب در گونه‌های دامی می‌پردازد. این رویکرد می‌‌تواند در تسهیل اجرای برنامه‌‌های اصلاح‌نژادی، ارتقای کارایی برنامه‌‌های تلقیح مصنوعی، بهبود کیفیت اسپرم‌‌های منجمد، حفظ ذخایر ژنتیکی دام‌های برتر و جلوگیری از انقراض گونه‌‌های در معرض خطر اهمیت کاربردی داشته باشد.
کلیدواژه‌ها

عنوان مقاله English

The Role of Berberine and Nano-Berberine as Antioxidants in Preserving the Quality and Freezability of Livestock Sperm: A Review Study

نویسندگان English

Mohammad Javad Karimi Sabet 1
Armin Towhidi 2
Parisa Nadri 1
1 Postdoctoral Researcher in Animal Physiology, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Alborz, Iran
2 Professor in Animal Physiology, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Alborz, Iran
چکیده English

During sperm cryopreservation, oxidative stress is recognized as one of the major factors contributing to reduced sperm motility, viability, membrane integrity, and ultimately fertilizing capacity. Compared with other cell types, sperm cells are particularly vulnerable to oxidative damage due to their limited cytoplasmic volume, low levels of intracellular antioxidants, high content of polyunsaturated fatty acids in the plasma membrane, and pronounced susceptibility to lipid peroxidation. The supplementation of antioxidants into semen extenders has been proposed as an effective strategy to mitigate cryoinjury and cold shock during the freezing–thawing process; however, the stability and bioavailability of these compounds may be compromised during cryopreservation. Berberine, a plant-derived alkaloid with potent antioxidant properties, is limited in practical applications due to its low bioavailability. In this context, berberine-loaded nanoparticles (nano-berberine) have emerged as a promising approach to enhance stability, cellular uptake, and protective efficacy against oxidative stress. Accordingly, the present review aims to summarize and critically evaluate the antioxidant effects of berberine and nano-berberine in semen cryopreservation media, with particular emphasis on their roles in improving post-thaw sperm quality in animal species. This strategy may contribute to the advancement of selective breeding programs, improvement of artificial insemination efficiency, enhancement of frozen semen quality, preservation of valuable genetic resources, and conservation of endangered animal species.

کلیدواژه‌ها English

Antioxidants
Berberine
Nano-berberine
Oxidative stress
Sperm cryopreservation
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