Gene transfer methods and use of primary germ cells and spermatogonia stem cells in the production of transgenic poultry; with an approach in the fields of genetics, medicine, and biopharmaceutical

Document Type : Review Article

Authors

1 M.Sc. of Animal Physiology, Department of Animal Science, College of Agriculture and Natural Resources at the University of Tehran, Karaj, Iran

2 Associate Professor of Animal Physiology, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Ph.D. Student of Animal and Poultry Breeding & Genetics, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Abstract

With the advent of molecular genetics, molecular tools, and bioinformatics, it became possible to determine the genetic nature of desirable traits in different animal species, and with the introduction of livestock and poultry breeding science, a new approach in breeding strategies became possible. Extensive breeding for traits such as increased growth rate and increased number of eggs produced in a period has reduced reproductive performance and disease resistance. Therefore, the use of transgenic animals especially poultry as a superior genetic model in genetic and physiological studies can be effective. The aim of this study was to introduce different methods of gene transfer, especially the two applied methods of using primary germ cells and spermatogonia stem cells in gene transfer to produce transgenic poultry as a superior genetic model in genetic, medical, and pharmaceutical studies. There are several methods for gene transfer and transgenic animal production of which primary germ cells and spermatogonia stem cells are the most commonly used ones. Primary germ cells are precursors of sperm and eggs that are programmed to transmit genetic and epigenetic information to future generations. Spermatogonia stem cells produce sperm through the process of spermatogenesis. As a result, the science of biotechnology and genome editing is advancing rapidly with increasing food production and food safety, as well as widespread application in medicine and pharmaceutical science in the world's growing population, and it can be used to develop breeding goals in various animal species. It can be used to develop resistance to infectious diseases and various geographical conditions; the applications of biotechnology in livestock and poultry breeding strategies are endless.

Keywords

References

Ashtiani, S.K., Nasr-Esfahani, M.H., Hosseini, S.M., Moulavi, F., Hajian, M., and et al. (2008). “Royana: successful experience in cloning the sheep.” Yakhteh, 10, 193-200.
Brackett, B.G., Baranska, W., Sawicki, W. and Koprowski, H. (1971). “Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization.” Proceedings of the National Academy of Sciences, 68(2), 353-357.
Brinster, R.L., and Zimmermann, J.W. (1994). “Spermatogenesis following male germ-cell transplantation.” Proceedings of the National Academy of Sciences, 91, 11298-11302.
Clark, J. and Whitelaw, B. (2003). “A future for transgenic livestock.” Nature Reviews Genetics, 4(10), 825-833.
Divya, D., Shukla, R., Chatterjee, R.N., Sagar, G., Prasad, A.R., and et al. (2021). “Production of Transgenic Chimeric Chicken from Cryopreserved Primordial Germ Cells and its Validation by Developing shRNA Transgenic Chicken Chimera.” Research Square, 1-11.
Fässler, R. (2004). “Lentiviral transgene vectors.” EMBO Reports, 5 (1): 28-29.
Fouad, A.M., and El-Senousey, H.K. (2014). “Nutritional factors affecting abdominal fat deposition in poultry: a review.” Asian-Australasian Journal of Animal Sciences, 27(7), 1057-1068.
Ghadimi, F., Shakeri, M., Zhandi, M., Zaghari, M., Piryaei, A., and et al. (2017). “Different approaches to establish infertile rooster.” Animal Reproduction Science, 186, 31-36.
Ghafouri, F., Sadeghi, M., Bahrami, A., and Miraei Ashtiani, S. (2020). “Identification of genes affecting the amount of abdominal fat in broiler chickens ‎using microarray and RNA sequencing data.” Iranian Journal of animal Science, 50(4), 259-269.
Giassetti, M.I., Ciccarelli, M., and Oatley, J.M. (2019). “Spermatogonial Stem Cell Transplantation: Insights and Outlook for Domestic Animals.” Annual Review of Animal Biosciences, 7, 385-401.
Gordon, J.W., Scangos, G.A., Plotkin, D.J., Barbosa, J.A. and Ruddle, F.H. (1980). “Genetic transformation of mouse embryos by microinjection of purified DNA.” Proceedings of the National Academy of Sciences, 77(12), 7380-7384.
Hammer, R.E., Pursel, V.G., Rexroad, C.E., Wall, R.J., Bolt, D.J., and et al. (1985). “Production of transgenic rabbits, sheep and pigs by microinjection.” Nature, 315, 680-683.
Han, J.Y. (2009). “Germ cells and transgenesis in chickens.” Comparative Immunology, Microbiology and Infectious Diseases, 32, 61-80.
Han, J.Y., Cho, H.Y., Kim, Y.M., Park, K.J., Jung, K.M. and et al. (2020). “Production of quail (Coturnix japonica) germline chimeras by transfer of Ficoll-enriched spermatogonial stem cells.” Theriogenology, 154, 223-231.
Harel-Markowitz, E., Gurevich, M., Shore, L.S., Katz, A., Stram, Y. and et al. (2009). “Use of sperm plasmid DNA lipofection combined with REMI (restriction enzyme-mediated insertion) for production of transgenic chickens expressing eGFP (enhanced green fluorescent protein) or human follicle-stimulating hormone.” Biology of Reproduction, 80(5), 1046-1052.
Herrid, M., Vignarajan, S., Davey, R., Dobrinski, I., and Hill, J.R. (2006). “Successful transplantation of bovine testicular cells to heterologous recipients.” Reproduction, 132, 617-624.
Houdebine, L.-M., (2002). “Animal transgenesis: recent data and perspectives.” Biochimie, 84, 1137-1141.
Jiang, Z.Q., Wu, H.Y., Tian, J., Li, N. and Hu, X.X. (2020). “Targeting lentiviral vectors to primordial germ cells (PGCs): An efficient strategy for generating transgenic chickens.” Zoological Research, 41(3), 281.
Kanatsu-Shinohara, M., Kato, M., Takehashi, M., Morimoto, H., Takashima, S., and et al. (2008). “Production of transgenic rats via lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells.” Biology of Reproduction, 79, 1121-1128.
Li, B., Sun, G., Sun, H., Xu, Q., Gao, B., and et al. (2008). “Efficient generation of transgenic chickens using the spermatogonial stem cells in vivo and ex vivo transfection.” Science in China Series C: Life Sciences, 51, 734-742.
Miao, X.-Y. (2011). “Production of transgenic animals using spermatogonial stem cells.” Agricultural Sciences in China, 10, 762-768.
Min, S., Qing, S.Q., Hui, Y.Y., Zhi, F., Rong, Q.Y., and et al. (2011). “Generation of antiviral transgenic chicken using spermatogonial stem cell transfected in vivo.” African Journal of Biotechnology, 10(70), 15678-15683.
Motono, M., Yamada, Y., Hattori, Y., Nakagawa, R., Nishijima, K.-i., and et al. (2010). “Production of transgenic chickens from purified primordial germ cells infected with a lentiviral vector.” Journal of Bioscience and Bioengineering, 109, 315-321.
Mozdziak, P.E., and Petitte, J.N. (2004). “Status of transgenic chicken models for developmental biology.” Developmental Dynamics, 229, 414-421.
Mukae, T., Okumura, S., Watanobe, T., Yoshii, K., Tagami, T. and et al. (2021). “Production of Recombinant Monoclonal Antibodies in the Egg White of Gene-Targeted Transgenic Chickens.” Genes, 12(1), 38.
Nakamura, Y., Usui, F., Ono, T., Takeda, K., Nirasawa, K., and et al. (2010). “Germline replacement by transfer of primordial germ cells into partially sterilized embryos in the chicken.” Biology of Reproduction, 83, 130-137.
Nakamura, Y., Yamamoto, Y., Usui, F., Mushika, T., Ono, T., and et al. (2007). “Migration and proliferation of primordial germ cells in the early chicken embryo.” Poultry Science, 86, 2182-2193.
Park, T.S., Jeong, D.K., Kim, J.N., Song, G.H., Hong, Y.H., and et al. (2003). “Improved germline transmission in chicken chimeras produced by transplantation of gonadal primordial germ cells into recipient embryos.” Biology of Reproduction, 68, 1657-1662.
Perry, A.C., Wakayama, T., Kishikawa, H., Kasai, T., Okabe, M., and et al. (1999). “Mammalian transgenesis by intracytoplasmic sperm injection.” Science, 284, 118.
Rasouli-Gharehsaghal, K., Shakeri, M., Zhandi, M., Amini, H.R., Yousefi, A.R. and Asadirad, M., (2020). Improvement of in vitro proliferation of cockerel spermatogonial stem cells using different combinations of growth factors. British Poultry Science, 61(6), 660-668.

Files

Share

How to cite

Statistics