Investigating polymorphism of TNF-alpha genes and their effect on tail fat trait in Makoi, Qezel and Romanov sheep

Document Type : Scientific-Extensional Article

Authors

1 M.Sc. Student of Animal and Poultry Breeding & Genetics, Department of Animal Science, Faculty of Agriculture at the Urmia University, West Azerbaijan, Urmia, Iran

2 Associate Professor of Animal and Poultry Breeding & Genetics, Department of Animal Science, Faculty of Agriculture at the Urmia University, West Azerbaijan, Urmia, Iran

Abstract

Tumor necrosis factor alpha (TNF-alpha) is a pro-inflammatory cytokine that is particularly important in regulating the immune response, including macrophage activity and cell death. The present research was conducted in order to investigate the presence or absence of polymorphism in Qezel, Makoi and Romanov sheep by using the polymerase chain reaction (PCR) method and the spatial form difference of single strands (SSCP). For this purpose, to determine the TNF-ALPHA gene polymorphism in Makoi, Qezel and Romanov sheep population, blood was taken from 50 Makoi sheep, 50 Qezel sheep and 50 Romanov sheep. An optimized saline method was used for DNA extraction. The quality of DNA was checked using 1% agarose gel and its quantity was checked with a spectrophotometer. A thermocycler was used to amplify a fragment of the TNF-alpha gene. Polymerase chain reaction was performed to amplify the 273 bp fragment of exon number 4 of TNF-alpha gene. Genotyping was done on a 12% polyacrylamide gel and the samples were placed in a thermocycler, finally they were placed in a polyacrylamide gel placed in a vertical electrophoresis machine. The appearance and coloring stage was done by silver nitrate solution. The analysis and analysis of the gene patterns observed in the TNF-alpha gene on acrylamide showed that the patterns created in Qezel, Romanov and Makoi breeds were monomorphic, which indicates the absence of polymorphism in this locus.

Keywords

References

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Professional Journal of Domestic
Volume 24, Issue 1 - Serial Number 28
In Progress
June 2024
Pages 39-46

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